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hepg2 human hepatic cells  (BPS Bioscience)


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    BPS Bioscience hepg2 human hepatic cells
    Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in <t>HepG2-ARE</t> cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.
    Hepg2 Human Hepatic Cells, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepg2 human hepatic cells/product/BPS Bioscience
    Average 94 stars, based on 49 article reviews
    hepg2 human hepatic cells - by Bioz Stars, 2026-06
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    1) Product Images from "Hydroethanolic Extract of Polygonum aviculare L. Mediates the Anti-Inflammatory Activity in RAW 264.7 Murine Macrophages Through Induction of Heme Oxygenase-1 and Inhibition of Inducible Nitric Oxide Synthase."

    Article Title: Hydroethanolic Extract of Polygonum aviculare L. Mediates the Anti-Inflammatory Activity in RAW 264.7 Murine Macrophages Through Induction of Heme Oxygenase-1 and Inhibition of Inducible Nitric Oxide Synthase.

    Journal: Plants (Basel, Switzerland)

    doi: 10.3390/plants13233314

    Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in HepG2-ARE cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.
    Figure Legend Snippet: Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in HepG2-ARE cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.

    Techniques Used: Activation Assay, Activity Assay, Control



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    Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in <t>HepG2-ARE</t> cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.
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    Image Search Results


    Preclinical evaluation of QUINOLAM on metabolic and cellular endpoints in HepG2 cells. ( A ) Cell viability assessed by MTT assay after 24 h exposure to increasing concentrations of QUINOLAM (0.003 to 1.6 mg/mL). No cytotoxic effects were observed up to 1.6 mg/mL. SDS (1 mg/mL) was used as a positive control. ( B ) Glucose uptake measured using the 2-NBDG fluorescent analog following 24 h treatment with QUINOLAM at 0.5, 1.0, and 2.5 mg/mL. A dose-dependent increase in glucose uptake was observed, with significant enhancement at 2.5 mg/mL ( p < 0.01 vs. control). ( C ) LDL receptor (LDL-R) protein expression determined by ELISA after 24 h exposure to QUINOLAM. Treatments with 1.0 and 2.5 mg/mL significantly upregulated LDL-R levels compared to untreated controls ( p < 0.05). ( D ) Antioxidant activity of QUINOLAM evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. QUINOLAM displayed strong antioxidant potential in a dose-dependent manner, with significant increases in TEAC values at 0.1 and 0.2 mg/mL (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control). All data are expressed as mean ± standard deviation from three independent experiments.

    Journal: Medicina

    Article Title: Retrospective Analysis of a Quince, Olive Leaf, and Amaranth Nutraceutical in Patients with Metabolic Syndrome

    doi: 10.3390/medicina61091638

    Figure Lengend Snippet: Preclinical evaluation of QUINOLAM on metabolic and cellular endpoints in HepG2 cells. ( A ) Cell viability assessed by MTT assay after 24 h exposure to increasing concentrations of QUINOLAM (0.003 to 1.6 mg/mL). No cytotoxic effects were observed up to 1.6 mg/mL. SDS (1 mg/mL) was used as a positive control. ( B ) Glucose uptake measured using the 2-NBDG fluorescent analog following 24 h treatment with QUINOLAM at 0.5, 1.0, and 2.5 mg/mL. A dose-dependent increase in glucose uptake was observed, with significant enhancement at 2.5 mg/mL ( p < 0.01 vs. control). ( C ) LDL receptor (LDL-R) protein expression determined by ELISA after 24 h exposure to QUINOLAM. Treatments with 1.0 and 2.5 mg/mL significantly upregulated LDL-R levels compared to untreated controls ( p < 0.05). ( D ) Antioxidant activity of QUINOLAM evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. QUINOLAM displayed strong antioxidant potential in a dose-dependent manner, with significant increases in TEAC values at 0.1 and 0.2 mg/mL (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control). All data are expressed as mean ± standard deviation from three independent experiments.

    Article Snippet: All in vitro experiments were conducted using the HepG2 human hepatic cell line (ATCC, Manassas, VA, USA).

    Techniques: MTT Assay, Positive Control, Control, Expressing, Enzyme-linked Immunosorbent Assay, Antioxidant Activity Assay, Standard Deviation

    Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in HepG2-ARE cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.

    Journal: Plants (Basel, Switzerland)

    Article Title: Hydroethanolic Extract of Polygonum aviculare L. Mediates the Anti-Inflammatory Activity in RAW 264.7 Murine Macrophages Through Induction of Heme Oxygenase-1 and Inhibition of Inducible Nitric Oxide Synthase.

    doi: 10.3390/plants13233314

    Figure Lengend Snippet: Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in HepG2-ARE cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.

    Article Snippet: HepG2 human hepatic cells harboring the construct containing the antioxidant response element (ARE) sequence following the luciferase-encoding gene were purchased from BPS Bioscience (#60513).

    Techniques: Activation Assay, Activity Assay, Control